Laboratorio di Spettroscopia µ-Raman

Referente
Davide Novella

Attività

The Raman effect is based on light interacting with the chemical bonds of a sample. Vibrations of chemical bonds interacting with photons produce specific energy shifts in the back scattered light that appear in a Raman spectrum. The Raman spectrum is therefore unique for each chemical composition and like a “fingerprint” capable to provide qualitative and quantitative information of the investigated material. Crucially, it is a non-invasive and non-destructive methodology and, generally, it does not require complex sample preparation.

The confocal microscope systems provide depth resolution and a strongly reduced background signal and facilitate the generation of depth profiles and 3D images with exceptional spectral and spatial resolution. With this technique, the specimen can be analysed in segments along optical axes and depth profiles or 3D images can be generated.

With the TrueSurface application of the Witec system the surface of the sample can be kept constantly in focus during the entire acquisition, without the topography roughness. The optical sensor controls the distance between the objective and the sample surface with sub-micrometre precision. Thus, any variation occurring during measurements with long integration times is compensated for, resulting in perfectly sharp and detailed images

Strumentazione

Raman WITec Alpha 300 R

Zeiss microscope attached (10x, 20x, 50x LD, 50x, 100x obj.s)
532 nm laser - grating 300 and 1800 g/mm
785 nm laser - grating 300 and 1200 g/mm
Spectrometer 1: UHTS 600 (VIS) (with 2 spectrographs)
Spectrometer 2: UHTS 400 (NIR) (with 2 spectrographs)
Motorised x-y-sample scanning stage for confocal Raman imaging,
TrueSurface microscopy and automated measurements routines ( 50x50 mm² travel range in reflection builds; Software controlled)
PC Windows with 2 software: Control Five (operate system) + Project Five (data treatment)

Sede

Dipartimento di Geoscienze - via G. Gradenigo, 6 - 35131 Padova (Italy)

Primo piano: 01-050

Contatti

RESPONSABILE: Dott. Davide Novella - Tel. +39 0498279158 E-mail: davide.novella@unipd.it

TECNICO: Dott.ssa Lisa Santello - Tel. +39 0498279131 E-mail: lisa.santello@unipd.it

Norme di accesso
Regolamento Raman Lab Gennaio 2023.pdf